anticalbindind9k antibody  (Santa Cruz Biotechnology)

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: MMQ and GH3 cells were treated with E2 (1 nM) or E2 + ICI182780 (1 μM) and transfected with luc-ERE, then ( A,B ) the luciferase reporter gene assay was carried out to evaluate the effects of E2 and ERs on CaBP-9k transcription (* P <0.05, compared with NC group, # P <0.05, compared with E2 group).

Article Snippet: After 1 h of incubation with 50 μl protein A, the supernatants were incubated with anti-CaBP-9k antibody (No. sc-74462, Santa Cruz) at 4°C overnight.

Techniques: Transfection, Luciferase, Reporter Gene Assay

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: ( A,B ) MMQ and GH3 cells were treated with 0, 0.1, 1 and 10 nmol/l E2 for 24 h, then the cells were collected and submitted to (A,B) Western blotting assay to test the protein expression levels of CaBP-9k, ERα and ERβ (* P <0.05). ( C,D ) GH3 and MMQ cells were treated with 300 nM AZD9496 for 1 h prior to 1 nM E2 treatment, then the cells were harvested and subjected to Western blotting to test the protein expressions of CaBP-9k, ERα and ERβ (* ,+ P <0.05, compared with NC group; # P<0.05, compared with E2 group).

Article Snippet: After 1 h of incubation with 50 μl protein A, the supernatants were incubated with anti-CaBP-9k antibody (No. sc-74462, Santa Cruz) at 4°C overnight.

Techniques: Western Blot, Expressing

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: ( A ) IP assay was carried out to analyze the interaction between CaBP-9k protein and ERα/ERβ protein in GH3 cells. ( B ) The subcellular location of CaBP-9k and ERα proteins in GH3 cells were determined by immunofluorescence.

Article Snippet: After 1 h of incubation with 50 μl protein A, the supernatants were incubated with anti-CaBP-9k antibody (No. sc-74462, Santa Cruz) at 4°C overnight.

Techniques: Immunofluorescence

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: ( A ) The knockdown efficiency of sh-ERα in GH3 and MMQ cells was determined by Western blotting assay. MMQ and GH3 cells were treated with E2, E2+AZD9496, or E2+sh-ERα, then cells were collected for the following assays. ( B,C ) CCK-8 assay used for cell viability detection. ( D,E ) Flow cytometry used for cell apoptosis detection. ( F,H ) Western blotting analysis used for detection of the protein expression of CaBP-9k (* P <0.05, compared with NC group; # P <0.05, compared with E2 group).

Article Snippet: After 1 h of incubation with 50 μl protein A, the supernatants were incubated with anti-CaBP-9k antibody (No. sc-74462, Santa Cruz) at 4°C overnight.

Techniques: Knockdown, Western Blot, CCK-8 Assay, Flow Cytometry, Expressing

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: ( A ) The knockdown efficiency of sh-CaBP-9k was determined by Western blotting assay in both MMQ and GH3 cell lines. Then, MMQ and GH3 cells were treated with E2, sh-CaBP-9k or E2+sh-CaBP-9k and then were submitted to the following assays. ( B,C ) CCK-8 assay was used to detect cell viability. ( D,E ) Flow cytometry was performed to detect cell apoptosis (* P <0.05, compared with NC group, # P <0.05, compared with E2 group).

Article Snippet: After 1 h of incubation with 50 μl protein A, the supernatants were incubated with anti-CaBP-9k antibody (No. sc-74462, Santa Cruz) at 4°C overnight.

Techniques: Knockdown, Western Blot, CCK-8 Assay, Flow Cytometry

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: MMQ and GH3 cells were treated with E2 (1 nM) or E2 + ICI182780 (1 μM) and transfected with luc-ERE, then ( A,B ) the luciferase reporter gene assay was carried out to evaluate the effects of E2 and ERs on CaBP-9k transcription (* P <0.05, compared with NC group, # P <0.05, compared with E2 group).

Article Snippet: Next, the membrane was blocked with 5% non–fat milk for 1 h at room temperature, and incubated with the primary antibodies CaBP-9k (No. sc-74462, Santa Cruz, CA, U.S.A.), ERα (No. ab32063, Abcam, MA, U.S.A.), ERβ (No. sc-53494, Santa Cruz) or GAPDH (Proteintech, Hubei, China) overnight at 4°C.

Techniques: Transfection, Luciferase, Reporter Gene Assay

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: ( A,B ) MMQ and GH3 cells were treated with 0, 0.1, 1 and 10 nmol/l E2 for 24 h, then the cells were collected and submitted to (A,B) Western blotting assay to test the protein expression levels of CaBP-9k, ERα and ERβ (* P <0.05). ( C,D ) GH3 and MMQ cells were treated with 300 nM AZD9496 for 1 h prior to 1 nM E2 treatment, then the cells were harvested and subjected to Western blotting to test the protein expressions of CaBP-9k, ERα and ERβ (* ,+ P <0.05, compared with NC group; # P<0.05, compared with E2 group).

Article Snippet: Next, the membrane was blocked with 5% non–fat milk for 1 h at room temperature, and incubated with the primary antibodies CaBP-9k (No. sc-74462, Santa Cruz, CA, U.S.A.), ERα (No. ab32063, Abcam, MA, U.S.A.), ERβ (No. sc-53494, Santa Cruz) or GAPDH (Proteintech, Hubei, China) overnight at 4°C.

Techniques: Western Blot, Expressing

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: ( A ) IP assay was carried out to analyze the interaction between CaBP-9k protein and ERα/ERβ protein in GH3 cells. ( B ) The subcellular location of CaBP-9k and ERα proteins in GH3 cells were determined by immunofluorescence.

Article Snippet: Next, the membrane was blocked with 5% non–fat milk for 1 h at room temperature, and incubated with the primary antibodies CaBP-9k (No. sc-74462, Santa Cruz, CA, U.S.A.), ERα (No. ab32063, Abcam, MA, U.S.A.), ERβ (No. sc-53494, Santa Cruz) or GAPDH (Proteintech, Hubei, China) overnight at 4°C.

Techniques: Immunofluorescence

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: ( A ) The knockdown efficiency of sh-ERα in GH3 and MMQ cells was determined by Western blotting assay. MMQ and GH3 cells were treated with E2, E2+AZD9496, or E2+sh-ERα, then cells were collected for the following assays. ( B,C ) CCK-8 assay used for cell viability detection. ( D,E ) Flow cytometry used for cell apoptosis detection. ( F,H ) Western blotting analysis used for detection of the protein expression of CaBP-9k (* P <0.05, compared with NC group; # P <0.05, compared with E2 group).

Article Snippet: Next, the membrane was blocked with 5% non–fat milk for 1 h at room temperature, and incubated with the primary antibodies CaBP-9k (No. sc-74462, Santa Cruz, CA, U.S.A.), ERα (No. ab32063, Abcam, MA, U.S.A.), ERβ (No. sc-53494, Santa Cruz) or GAPDH (Proteintech, Hubei, China) overnight at 4°C.

Techniques: Knockdown, Western Blot, CCK-8 Assay, Flow Cytometry, Expressing

Journal: Bioscience Reports

Article Title: 17β-estradiol binding to ERα promotes the progression of prolactinoma through estrogen-response element-induced CaBP-9k up-regulation

doi: 10.1042/BSR20191330

Figure Lengend Snippet: ( A ) The knockdown efficiency of sh-CaBP-9k was determined by Western blotting assay in both MMQ and GH3 cell lines. Then, MMQ and GH3 cells were treated with E2, sh-CaBP-9k or E2+sh-CaBP-9k and then were submitted to the following assays. ( B,C ) CCK-8 assay was used to detect cell viability. ( D,E ) Flow cytometry was performed to detect cell apoptosis (* P <0.05, compared with NC group, # P <0.05, compared with E2 group).

Article Snippet: Next, the membrane was blocked with 5% non–fat milk for 1 h at room temperature, and incubated with the primary antibodies CaBP-9k (No. sc-74462, Santa Cruz, CA, U.S.A.), ERα (No. ab32063, Abcam, MA, U.S.A.), ERβ (No. sc-53494, Santa Cruz) or GAPDH (Proteintech, Hubei, China) overnight at 4°C.

Techniques: Knockdown, Western Blot, CCK-8 Assay, Flow Cytometry